Status of Genotyping Approaches for Polyploid Crops (2018)

Collaborative Development of Tools for Successful Variant Calling in Challenging Polyploid Backgrounds

This presentation begins with an overview of the solutions from Thermo Fisher Scientific before discussing collaborations, polyploid genotyping, and GBS Solutions. It then concludes by looking at the future advances possible in polyploid genotyping.

Collaborative Development of Tools for Successful Variant Calling in Challenging Polyploid Backgrounds Video

Collaborative Development of Tools for Successful Variant Calling in Challenging Polyploid Backgrounds Slides

 

From Discovery to Decision: Genotyping Solutions for Polyploids

This presentation discusses the genomics continuum, shows the value of arrays, and looks at the Infinium-based products for polyploid species. It then talks about the new technology; Infinium XT before finishing with a summary of genotyping as a whole.

From Discovery to Decision Video

From Discovery to Decision Slides

 

Targeted Sequencing of Polyploids: Best Practices

This presentation discusses the definition of targeted sequencing and in-solution target capture. It then talks about the advantages and applications for targeted sequencing, as well as the probe design. It finishes by discussing the genotyping techniques.

Targeted Sequencing of Polyploids: Best Practices Video

Targeted Sequencing of Polyploids: Best Practices Slides

 

Polyploid Genetics in Kiwifruit and Potato

This presentation looks at the new polyploid projects being undertaken at The New Zealand Institute for Plant and Food research. It also discusses the genetics and polyploidy of green and gold kiwifruit, and takes a look at the linkage map and capture GBS.

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Polyploid Genetics in Kiwifruit and Potato Slides

 

Addressing Polyploidy in a GBS Pipeline for Minor/Orphan Crops Lacking a Reference Genome

This presentation discusses the aspects of genotyping by sequencing, or GBS. It also talks about how the GBS SNP Calling Reference-Optional Pipeline, the GBS SNP CROP, works, and improvements with it.

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Addressing Polyploidy in a GBS Pipeline for Minor/Orphan Crops Lacking a Reference Genome Slides

 

DArTseq and DArTcap Technologies for Polyploids

This presentation goes over DArT PL and their mission of actively supporting more effective and sustainable uses of natural resources. It also discusses how the team works, the diversity of their client base, how and why the DArTseq works, and the crops that it has been used with.

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DArTseq and DArTcap Technologies for Polyploids Slides

 

Genotyping Approaches Discussion and Summary

This presentation looks at several genotyping methods, including simple sequence repeat, hydrolysis and FRET assays, and targeted sequencing. It then discusses the advantages and disadvantages of each, and compares them to each other.

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Discussion and Summary Slides